Analysis Note

ControlDeveloping striated muscle tissue

Application

Anti-Laminin α5 Antibody, clone 4C7 is an antibody against Laminin α5 for use in ELISA, IH & IP.

ELISA (50% maximal binding to human laminin): >1:11,000.

Immunofluorescence - tissue staining pattern is most consistent with that observed for laminin alpha5 in the mouse(Miner et al., 1997)

Affinity chromatography

Immunoprecipitation

Does not work for Western blotting

Optimal working dilutions must be determined by end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

UnPurified mouse ascites containing no preservatives.

Specificity

Reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin. This antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain [see Tiger, C.F. (1997) J. Biol. Chem. 272: 28590].